pcdna3 1 circrna mini vector (Addgene inc)
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Addgene inc
pcdna3 1 circrna mini vector
Pcdna3 1 Circrna Mini Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+circrna+mini+vector/pcDNA3%2E1(%2B)+CircRNA+Mini+Vector+(Plasmid+%2360648)/pm41634201-368-14-19
Average 96 stars, based on 247 article reviews
Pcdna3 1 Circrna Mini Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+circrna+mini+vector/pcDNA3%2E1(%2B)+CircRNA+Mini+Vector+(Plasmid+%2360648)/pm41634201-368-14-19
Average 96 stars, based on 247 article reviews
pcdna3 1 circrna mini vector - by Bioz Stars,
2026-09
96/100 stars
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Over Expression:Article Title: Tumor cell-intrinsic circular RNA circFNDC3B attenuates CD8 + T cells infiltration in non-small cell lung cancer Article Snippet: Fluorescence images were captured using a confocal laser scanner, and the data were analyzed using ImageJ software. .. To establish circFNDC3B, circFndc3b, TFII-I, and STAT1 overexpression plasmids, circFNDC3B, circFndc3b, TFII-I, and STAT1 cDNA were synthesized by TSINGKE (Wuhan, China) and then cloned into pcDNA3.1( Synthesized:Article Title: Tumor cell-intrinsic circular RNA circFNDC3B attenuates CD8 + T cells infiltration in non-small cell lung cancer Article Snippet: Fluorescence images were captured using a confocal laser scanner, and the data were analyzed using ImageJ software. .. To establish circFNDC3B, circFndc3b, TFII-I, and STAT1 overexpression plasmids, circFNDC3B, circFndc3b, TFII-I, and STAT1 cDNA were synthesized by TSINGKE (Wuhan, China) and then cloned into pcDNA3.1( Clone Assay:Article Title: Tumor cell-intrinsic circular RNA circFNDC3B attenuates CD8 + T cells infiltration in non-small cell lung cancer Article Snippet: Fluorescence images were captured using a confocal laser scanner, and the data were analyzed using ImageJ software. .. To establish circFNDC3B, circFndc3b, TFII-I, and STAT1 overexpression plasmids, circFNDC3B, circFndc3b, TFII-I, and STAT1 cDNA were synthesized by TSINGKE (Wuhan, China) and then cloned into pcDNA3.1( Article Title: Tumor Cell-Intrinsic Circular RNA CircFNDC3B Attenuates CD8+ T Cells Infiltration in Non-Small Cell Lung Cancer Article Snippet: Vector construction and cell transfection To establish circFNDC3B, circFndc3b, TF -I and STAT1 over-expression plasmids, circFNDC3B, circFndc3b, TF -I and STAT1 cDNA were synthesized by TSINGKE (Wuhan, China). .. Then they were cloned into pcDNA3.1( Article Title: A Novel Mechanism Linking Melatonin, Ferroptosis and Microglia Polarization via the Circodz3/HuR Axis in Subarachnoid Hemorrhage. Article Snippet: Subarachnoid hemorrhage (SAH) is a severe hemorrhagic disorder of the central nervous system with a high rate of disability and mortality.. The overall crude incidence of SAH is approximately 7.9 per 100,000 people, and SAH, accounting for ∼ 5% of all strokes, is the third common type of stroke [1, 2].. It is particularly concerning that the mortality rate for SAH can be as high as 50%, and even if patients survive, many of them remain long-term disability [3]. Plasmid Preparation:Article Title: RNA editing enzyme ADAR2 regulates P-glycoprotein expression in murine breast cancer cells through the circRNA-miRNA pathway. Article Snippet: The following reagents and equipment were used: PrimeSTAR MAX DNA polymerase (Takara Bio Inc., Shiga, Japan), pGL4.13 luciferase reporter vector, pmirGLO vector, pRL-TK vector, Dual-Luciferase reporter assay system, GloMax 20/20 Luminometer (Promega, Madison, WI), miRIDIAN microRNA Mimic Negative Control #1 and mmu-miR-195a-3p (Horizon Discovery, Cambridge, UK), Lipofectamine LTX reagent, and Lipofectamine 2000 reagent (Invitrogen; Life Technologies). .. The pcDNA3.1( Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the Article Title: CircLIFRSA/miR-1305/PTEN axis attenuates malignant cellular processes in non-small cell lung cancer by regulating AKT phosphorylation Article Snippet: Human lung adenocarcinoma cell lines (A549, NCI-H1299), as well as normal human bronchial epithelial cells (HBE), were maintained in RPMI-1640 medium (Hyclone, USA) supplemented with 10% fetal bovine serum (FBS) (PAN, Germany) under conditions of 37 °C and 5% CO 2 . .. Transfections were performed using either circLIFRSA siRNA (GenePharma, China) alongside its corresponding negative control RNA (siRNA NC), or a recombinant plasmid designed to overexpress circLIFRSA in conjunction with the empty pcDNA3.1( Article Title: CircNFATc3 promotes Fetal Hemoglobin induction by regulating let-7b/GATA2 axis Article Snippet: Images of the stained cells were taken using Nikon TE2000 microscope equipped with a digital camera. .. The pcDNA3.1( Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer. Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the pcDNA3.1( Polymerase Chain Reaction:Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer. Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the pcDNA3.1( Purification:Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer. Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the pcDNA3.1( Agarose Gel Electrophoresis:Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the Article Title: RNA sequencing reveals differential expression of circular RNAs in human small cell lung cancer. Article Snippet: .. The PCR product was purified from agarose gel, restriction digested, and ligated into the pcDNA3.1( Transfection:Article Title: CircLIFRSA/miR-1305/PTEN axis attenuates malignant cellular processes in non-small cell lung cancer by regulating AKT phosphorylation Article Snippet: Human lung adenocarcinoma cell lines (A549, NCI-H1299), as well as normal human bronchial epithelial cells (HBE), were maintained in RPMI-1640 medium (Hyclone, USA) supplemented with 10% fetal bovine serum (FBS) (PAN, Germany) under conditions of 37 °C and 5% CO 2 . .. Transfections were performed using either circLIFRSA siRNA (GenePharma, China) alongside its corresponding negative control RNA (siRNA NC), or a recombinant plasmid designed to overexpress circLIFRSA in conjunction with the empty pcDNA3.1( Negative Control:Article Title: CircLIFRSA/miR-1305/PTEN axis attenuates malignant cellular processes in non-small cell lung cancer by regulating AKT phosphorylation Article Snippet: Human lung adenocarcinoma cell lines (A549, NCI-H1299), as well as normal human bronchial epithelial cells (HBE), were maintained in RPMI-1640 medium (Hyclone, USA) supplemented with 10% fetal bovine serum (FBS) (PAN, Germany) under conditions of 37 °C and 5% CO 2 . .. Transfections were performed using either circLIFRSA siRNA (GenePharma, China) alongside its corresponding negative control RNA (siRNA NC), or a recombinant plasmid designed to overexpress circLIFRSA in conjunction with the empty pcDNA3.1( Recombinant:Article Title: CircLIFRSA/miR-1305/PTEN axis attenuates malignant cellular processes in non-small cell lung cancer by regulating AKT phosphorylation Article Snippet: Human lung adenocarcinoma cell lines (A549, NCI-H1299), as well as normal human bronchial epithelial cells (HBE), were maintained in RPMI-1640 medium (Hyclone, USA) supplemented with 10% fetal bovine serum (FBS) (PAN, Germany) under conditions of 37 °C and 5% CO 2 . .. Transfections were performed using either circLIFRSA siRNA (GenePharma, China) alongside its corresponding negative control RNA (siRNA NC), or a recombinant plasmid designed to overexpress circLIFRSA in conjunction with the empty pcDNA3.1( |